Contents of wells were pipetted up and down several times to mix. Microplate is validated by molecular devices for the FLIPR reader. The VersaMax reads 96-well microplates. /well integration in 96-well luminescence mode and 0. com. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . Detergent Compatible Bradford assay. Growth medium was then replaced with fatty acid uptake solution. In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 03 pM. Cross-talk < 0. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. The EMax Plus reader measures flat and round 96-well microplates. Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Listing: #4772697. 0200-6263. Description. Paradigm: Delta mP. No accessories are needed and SoftMax® Pro Software. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 3. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. SpectraMax Microplate Readers. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Click on the crosshair and move it so that it is centered over the well image. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Affordable multi-mode microplate reader with . Microplate Reader, Molecular Devices Optimax, Refurbished. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. 19 H mm that meet microplate industry standards. This unlocks the transport slide. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. Flexibility to read a variety of plate formats. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. adjust the reader. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. 03 pM 384-well: 0. July 17, 2023. explore more. LVF Monochromator™ : Excitation and Emission. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. 96-well microplate; Immunoassay analyzer;VersaMax price. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. #ID3-STD) • L-Tryptophan (Sigma cat. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. Detection by Low noise PMT with simultaneous digital and analog modes. accurately over a wide dynamic range. While "plate reader" usually refers to the devices described. flat-bottom: 50-60 µl. For more sample throughput, both readers can be easily integrated into full roboticThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Fluorescence Microplate Reader Comparison Chart. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. tools to expand the boundaries of their research. or ValitaTiter Plus (Molecular Devices cat. Plate readers, also known as microplate readers or microplate photometers,. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. Materials. Determine DNA concentrations precisely and . Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. 39. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. iD5. With optimized reagents, option to operate the. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 500 USD. SOP Title: Use and. The concentration of total free thiols in the protein. StakMax. 0 500 1000 1500 2000 2500 3000 3500 time (sec. 2 nm Wavelength Bandpass 2. Transfer of an assay to 96-well microplate technology increases throughput significantly. ***Figure 1. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Instrument settings are listed in Table 1. The contents of the SpectraDrop Microplate kit depend on kit type. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. Published Dec 16, 2020 | Updated Dec 06, 2021. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) at 30 °C. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Omnia® Compatible Microplate Reader Documentation Version No. microplate . Plate reader instructions Computer. Three low volume 24-well bottom slides. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. #0310-5227) 96-well cell wash head (Molecular Devices Cat. 10. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. 300 µL of Detergent Compatible Bradford reagent was added to each well. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. 3. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 96, 384, 24, 12, 6 well types. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. 14: Average %CV. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 1 pM. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . Plates can put in the drawer both in landscape and portrait positions. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Read the microplate Step 1: Place the microplate in the reader. Jon Cuccui, Brendan W. Molecular Devices provides. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. #T-0254) Lysozyme (Sigma cat. 0200-6262. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. 5. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 7. DNA concentration (ng/mL) Average RFU. Molecular Devices i3x. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Monochromator, tunable in 1-nm increments. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 40266. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup. Detection modalities are shown in Table 1-1. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. Pick the best speed, sensitivity, and price for your needs. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Supplier: Molecular Devices. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Microplates . These models can no longer be repaired. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. The screw remains inside the instrument. Outer wells are aligned for proper viewing with FLIPR. Bioz Stars score: 86/100, based on 1 PubMed citations. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. for ValitaTiter Plus standards read on Molecular Devices readers. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Incubate the plate at 37 °C for approximately one hour. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. 7°C and the bottom to 36. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Visible absorbance -. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The device was able to achieve 3σ limits of detection of ca. Making it possible to perform kinetic analysis of solid. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. 747. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Read More. To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. Compare this item. Learn more about how absorbance is measured, and some key applications that utilize. Softmax Pro GxP Software. Paradigm: Delta mP. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. 03 pM. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 2600A October 2023. The Multiskan Sky microplate reader is an absorbance plate reader. 96-well flat clear bottom white TC-treated microplates (Corning cat. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. the iD5 reader’s filter system. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Molecular Devices M5 Plate Reader Plate Calibration Form . We would like to show you a description here but the site won’t allow us. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. Microplate luminescent reader assay for ERK activity. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. Monochromatic filter is equipped. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 3% in white 96- and. Figure 2-23: Bottle holder To install the bottle holder: 1. The eight-channel system delivers both high precision and speed when reading 96-well microplates for endpoint measurements, kinetic assays, and spectral scans. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. #N207) LAL Reagent Water (Lonza cat. Page: 1/4. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. SoftMax Pro Software’s SpectralThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. The CellTiter-Glo assay from Promega uses luciferase. In addition, the iD5 reader measures TRF and FP and. 96-well: 0. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. to each well. The system provides options for 20-, 40-,. 76 L x 85. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Fluorescent DNA quantitation. The color touch screen provides a visual user interface, making programming fast and intuitive. ZERO BIAS. Use black microplates. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 96- or 384-well microplate compatibility. Infinite 200 Pro Microplate Reader. 98 Bovine serum albumin mg/mL O. Create custom fluorescence assays without having to purchase expensive filter sets. No additional manuals are available. It features a modular design that allows users to add or change detection cartridges according to their needs. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. It also integrates with the powerful SoftMax Pro Software for. ± 0. 100 μL/well Steady-Luc working solution was added to the wells. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Read More. MycoAlert Reagent was added to each well and incubated at room temperature for 5. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Equipped with scanning, shaking and kinetics modules. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. 69: 85. #L6876) • Guanidine hydrochloride (Sigma cat. Fluorescence Microplate Reader Comparison Chart. Less background noise gives a better limit of detection and so a relative increase in usable signal. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. 2022 Harini Natarajan et al. Model:. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Instrument settings can be saved as a protocol file and used repeatedly for reading differentThe Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . 7°C (gradient heating design). the appropriate well of the microplate. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. The system provides automation for up to 50 microplates for easy walkaway automation. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. Optical System: High energy xenon flash lamp. For more sample throughput, the system can be. In addition, the iD5 reader measures TRF and FP and. 50 racks/case for 384-well (16 channel pipettor head. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. M5. Specifications for the SpectraMax L microplate reader. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. SpectraMax 250 User. Microplate readers use a vertical light path so the distance of the. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. We would like to show you a description here but the site won’t allow us. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. 1-color 96-well . The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Step 2: Launch SoftMax Pro Software. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 0 H x 60. #N207) LAL Reagent Water (Lonza cat. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The plate reader optics are tunable for. SpectraMax 250 User. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. 1536-well: 0. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Microplate Readers. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. , Sigma cat. Transfer of an assay to 96-well microplate technology increases throughput significantly. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. SoftMax Pro Software’s. 3. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 3 OD at 200 nm. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. NanoRacks added it to their line of commercial . Measure more accurately. All stand-alone instrument functions can be controlled using the software. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. . SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The reader adds up to three transfers of assay reagents from a 96- or 384. Flexible Microplate Format The GEMINI system allows you to select from 6-, 12-, 24-, 48-, 96- and 384-well microplate formats for your fluorometric measurements. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. # 0310-5218T). Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. Timothy Bolus, MPM, MBA, Compliance Program Manager. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Published Dec 16, 2020 | Updated Dec 16, 2020. ValitaTiter Plus 96-well (1 plate) VAL004. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 1 mL/well of media. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. Infinite 200 Pro Microplate Reader.